The patterns of GUS expression at a variety of temperatures were determined in transgenic Arabidopsis. The data revealed that a decrease in GUS gene expression after low temperature treatment (4°C) (Fig 4A, 4C and 4F), but an increase after the other treatments (Fig 4A, 4C, 4D and 4E).
This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.
Sequence statusi: Complete. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences. This information was obtained by Southern blot analysis of … The Tm value of GUS3-3 is 80 degrees C and increased by 25 degrees C above GUS-ck (55 degrees C). UniRef. 1995, Mathur e… The Escherichia coli β-glucuronidase-encoding gene gusA is useful as a reporter gene in a variety of organisms. Insertion of the Em 5'-untranslated region (+6 to +86) between the 35S promoter and the ATG of GUS results in a 10-fold increase in GUS activity in the absence of ABA. This means that the GUS coding sequence is under the direction of the controlling sequence of another gene. Receive the latest news, hot plasmids, discounts and more. composed of the upstream sequence of the HSP18.2 gene from Arabidopsis and the bacterial gene for p-glucuronidase (GUS) after Agrobacteriummediated transfer into Arabidopsis plants. ronidase (GUS) reporter gene, and the left border. & ORFs. Cyclic redundancy and other checksumsThe GUS gene is usually used in a gene fusion. For this exercise the GUS gene is under the control of the Cauliflower Mosaic Virus 35S promotor. Insertion of a promoterless reporter gene within a transcriptional unit of the genome may generate in situ gene fusions, allowing the identification of individual genes and their regulatory elements based on the distinct expression patterns of the tagged reporter gene (Teeri et al. Sequence archive. The 3' DNA sequence (15 kb) flanking the gus::nptII insert in the plant with GUS expression in the stigma was also isolated using a subgenomic library. A bidirectional promoter is an intergenic region of ~1–1.5 kb that regulates the flanking protein coding gene pair 1,2.Known for many years, BDPs have been identified in all kingdoms 3,4,5,6,7,8 The deduced amino acid sequence demonstrated that 11 amino acid was changed. A 2.8 kb HindIII fragment including approximately 1.2 kb of upstream sequence and genomic sequence corresponding to the first 306 amino acids encoded by the CycB1;1 gene was isolated. The human OSM (oncostatin M) gene is involved in regulation of cell growth. What do I need to know about the customs and importation process for my country?
Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
gus protein can be detected in the cytoplasm of nurse cells during the germarial stage and by stage 3 of oogenesis is punctate in the cytoplasm. A guide RNA was designed to target BglII site downstream start codon on the GUS gene. Binary Agrobacterium vector with a GUS reporter gene for plant transformation. A 50-base pair sequence (-152 to -103) fused 5' in either orientation to a truncated cauliflower mosaic virus promoter (35S) increases GUS activity threefold in the presence of ABA.
However UniProtKB may contain entries with identical sequences in case The blue zones in leaves were significantly narrowed, while the corresponding leaves were … Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))
,This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).
,This section is used to point to information related to entries and found in data collections other than UniProtKB.
,This section provides general information on the entry.
,This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. The GUS reporter system was used to detect the efficiency of the CLCrV-mediated VIGE system. Finding cDNA sequence for a gene. 1986, Koncz et al. If GUS assay results in the expression of GUS gene specifically in trichomes then the sequence that was identified through T-DNA insertional mutagenesis will be confirmed as promoter that is responsible for expression of GUS gene in trichomes. is extremely low.
Use text editor or plasmid mapping software to view sequence. Ethylene, a two-carbon olefin, is a volatile hormone in higher plants. The grey color shows GUS gene sequence. ,This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.
,This subsection of the Names and taxonomy section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.
,Information which has been imported from another database using automatic procedures.
Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present. GUS activity is induced by DEX in 14 independent T1 pOp6/rcoLhGR lines.GUS enzymatic activity measured in total leaf protein extracts from individuals segregating in T1 lines transformed with construct 17203 (a, e), 17610 (B, F) or 17613 (c, g).Individuals from a pZmUbi:GUS line were used as positive controls, and wild-type Kitaake rice plants (WT) were used as negative control (d). If you run into any problems registering, depositing, or ordering please contact us at [email protected] The version number for both the entry and the canonical sequence are also displayed.,This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (reviewed) or to the computer-annotated TrEMBL section (unreviewed).
, The European Molecular Biology Laboratory, State Secretariat for Education, Research and Innovation, hydrolase activity, hydrolyzing O-glycosyl compounds. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.,This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.
,This section provides information on the tertiary and secondary structure of a protein.
,This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.
,This subsection of the Family and Domains section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.
,Information which has been generated by the UniProtKB automatic annotation system, without manual validation.
As a senescing hormone, it promotes leaf-yellowing, climacteric fruit ripening, flower and leaf abscission. pBI121. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.,Information which has been generated by the UniProtKB automatic annotation system, without manual validation.
Sequence clusters. 3.3. Description of accessions available : MT-DR5::GUS is a transgenic plant produced by Dr. Jose Luis Garcia-Martinez (Universidad Politécnica de Valencia, Spain). How do I place an order? 1989, Lindsey et al. DNA Data Bank of Japan; a nucleotide sequence database, ProtoNet; Automatic hierarchical classification of proteins, MobiDB: a database of protein disorder and mobility annotations. Protein sets from fully sequenced genomes. What is virus associated DNA, and why do I have to order it? As we show below, similar operons are found only in other Enterobacteriaceae, including Salmonella , Klebsiella , Yersinia , and Shigella taxa, all of which are potential intestinal and systemic pathogens. Together, these elements define the GUS gene complex. Bean et al. Tightly linked to the gene that encodes murine beta-glucuronidase (GUS) are three GUS-specific regulatory elements. (C) Group of vectors constructed in head to head orientation similar to B series vectors except that a 254 bp sequence (triangle) derived from pCAMBIA1300 was inserted to separate the GUS reporter and its adjacent inverted promoter used for hptII gene expression. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'., Automatic assertion inferred from database entries,This subsection of the Names and taxonomy section provides information on the name(s) of the organism that is the source of the protein sequence.
,This subsection of the Names and taxonomy section shows the unique identifier assigned by the NCBI to the source organism of the protein.
It should be noted that while, in theory, two different sequences could This similarity in the results could be related to the abundant cis -acting elements in the −400 bp sequence (−2,164 to −1,765) and the −250 bp sequence (−584 to 234) of the promoter. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.
Protein sets from fully sequenced genomes. This website uses cookies to ensure you get the best experience. At stage 10 there is a transient accumulation of the protein at the posterior pole. Both vectors contain several unique restriction sites upstream from gus and the yeast CYC1 … Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. By continuing to use this site, you agree to the use of cookies. Use text editor or plasmid mapping software to view sequence. 2015 Dec 7. pii: pp.01246.2015. As a stress hormone, it is involved in biotic and abiotic stress responses of plants (Yang and Hoffman, 1984; Ge et al., 2000). The diverse applications of the GUS gene fusion systems (Gallagher, 1992) are based on the detection of the enzymatic activity of GUS in protein extracts or in tissues using fluorometric and histochemical assays respectively (Jefferson, 1987). with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018. have the same checksum value, the likelihood that this would happen It is useful for tracking sequence updates. Annotation systems. GUS protein was originally isolated from E. coli which is ubiquitous in the digestive system of vertebrates, including humans. The algorithm is described in the ISO 3309 standard. Automatic assertion inferred from database entriesi, Automatic assertion inferred from database entriesi, ExpressionAtlas, Differential and Baseline Expression, SWISS-MODEL Repository - a database of annotated 3D protein structure models, Database of comparative protein structure models,Information which has been generated by the UniProtKB automatic annotation system, without manual validation.
Systems, Research The results are shown below. It regulates cytokine production, and is involved in the maturation of foetal hepatocytes. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free … We'd like to inform you that we have updated our Privacy Notice to comply The nucleotide sequence analysis showed 99.2% homology between the GUS-ck gene from pBI121 and GUS3-3 gene. bp sequence 5′-CCTTT TGTCTC -3′, a 46-bp CaMV35S minimal promoter element, and a TMV leader sequence. recently published a report “Diagnostic gene sequencing panels: from design to report – a technical standard of the American College of Medical Genetics and Genomics (ACMG)” that provides technical standards for the design of gene panels. In addition to its stimulato… The sequence results that the orientation of 3′UTR_B11 was inverted repeat. β -Glucuronidase (GUS) is a very versatile reporter of gene expression that is frequently used in plant molecular biology. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.,This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). A variant of uncertain (or unknown) significance (VUS) is a genetic variant that has been identified through genetic testing but whose significance to the function or health of an organism is not known. Leading primers are indicated on the first line of each sequence. There is a problem with the plasmid I received. (D) Group of vectors constructed in head to tail orientation with GUS reporter upstream of the selectable marker.
A gene for a cytochrome P450 like protein was discovered on the minus DNA strand of the 3' sequence with a start codon approximately 6.5 kb from the T-DNA … You are using a version of browser that may not display all the features of this website. What is an MTA/Who is authorized to sign?The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) It also includes information pertinent to the sequence(s), including length and molecular weight. The reporter gene was the uidA gene of Escherichia coli that encodes the enzyme β-glucuronidase (GUS). Specific alleles of each regulatory element are associated with a specific GUS structural allele. Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Several types of gene-trapping systems that utilize random integration of reporter gene constructs into the genome have been developed (Springer 2000). Genome Fields, Pathways Learn more, Download our file to copy and paste plasmid data, Open collection of AAV data generously shared by scientists, Basic analysis for a user-entered sequence; includes restriction sites and map, Digital collection of empty plasmid backbones from publications and commercially available sources. vectorcontainingthewidelyusedE.coliuidAgene,encoding f3-glucuronidase (GUS) protein (11), as a transcriptional reporter gene coupled to different Chlamydomonaschloro-plast promoters. The 3′UTR_B11 insert was cloned from the recombinant pENTR-3′UTR_B11 vector into pANDA vector using LR Clonase enzyme, which is part of the Gateway cloning system (Invitrogen™, USA).
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